Journal: Journal of Proteome Research
Article Title: Plasma Proteomic Profiling Identifies a Six-Protein Panel for Grading and Predicting Intervertebral Disc Degeneration
doi: 10.1021/acs.jproteome.5c00754
Figure Lengend Snippet: Clinical characteristics of patients with different IDD grades. (A) Representative images of magnetic resonance imaging. (B) Representative images of H&E staining. (C) Representative images of Safranin O-Fast Green staining. (D) Western blot analysis of MMP3, MMP13, ADAMTS4, and ADAMTS7 expression in grade II and grade V NP tissues. (E) Quantification of Western blot analysis measured by relative levels of β-actin. * p -value < 0.05.
Article Snippet: The membrane was blocked with a 5% BSA solution for 1 h. Following this, the membrane was incubated overnight at 4 °C with specific primary antibodies, including: anti -β-actin (AC026, ABclonal); anti -MMP3 (mAb no. 14351, Cell Signaling Technology); anti -MMP13 (mAb no. 69926, Cell Signaling Technology); anti -ADAMTS4 (ER1903-31, HuaBio); and anti -ADAMTS7 (ER1903-33, HuaBio).
Techniques: Magnetic Resonance Imaging, Staining, Western Blot, Expressing